FastQCFastQC Report
Tue 27 Nov 2018
HG2CCAFXY_n01_mcb-s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CCAFXY_n01_mcb-s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75305429
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3310810.43965090485043257No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1136910.15097317883946984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG222850.017.30723145
CTGAACG343150.015.063785145
CCAGTCC338500.014.435432145
GATCATG1383950.012.923416145
CACGTCG168700.012.161858145
TGCCGTG451600.010.836235145
GAGCACC338900.010.71753145
CTCCAGG313450.09.783644145
AGATCAG1820100.09.511909145
CGTCGGG220650.07.8856206145
TCACCAC549000.07.7780643145
TGGGGGG2125100.07.72706461
ATGCCGG1368100.07.662651145
TCGCGTT445150.07.475407145
CGTCTGG292500.07.2870207145
CGGAAGC433250.07.145257145
TGCCGGG1121850.06.9987783145
TCTCGTT667750.06.4708443145
CGTCGTG390400.06.3881803145
GCACACC262450.06.353453145
Produced by FastQC (version 0.11.5)