FastQCFastQC Report
Tue 27 Nov 2018
HG2CCAFXY_n01_mcb-s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG2CCAFXY_n01_mcb-s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42288003
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1265390.2992314392334866No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG474070.11210508096114163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG232600.025.246832145
ATGCCGG493950.023.49849145
CCAATCG351750.023.496452145
CGTCTTG342950.021.414612145
AGTCACG114250.021.004065145
CACACGG120350.018.31298145
TCACTGC353850.016.042576145
CTGAACG182200.015.717385145
CACGTCG85750.015.641168145
TGGGGGG479650.014.8275871
GTCTTCG449250.013.168419145
ACCAATG621100.011.60263145
GAGCACC181250.010.07985145
CTTCTGG375100.09.625291145
TCTCGTG143300.09.207819145
CTCCAGG164850.09.015638145
CCAGTCC149300.08.886338145
TTCTGCG472600.08.744052145
CGTCTGG125050.08.464489145
GACCAAG747950.08.38446145
Produced by FastQC (version 0.11.5)