FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20062148
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10463895.215737616929155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG41900.024.7404695
ACGAGAT108350.014.517977
CGGGAAC101850.014.4923262
GGAACGT106300.013.5077024
GATCCTG56100.013.1802375
GAACGTA123750.012.9459225
CGAGGGA98700.012.9261938
CCCCTGA58000.011.878054
GTATTCA134300.011.8746769
CCGGGAA128600.011.71721
GTAGATA127950.011.5592145
CCGTATA42100.011.193584145
CCCTGAG84550.010.88870055
TATTCAC139250.010.8278759
CGTGTAT72050.010.464952145
GGGAACG165000.010.4300013
TTTGATC90350.010.301132
ATCCCCT70050.09.9647452
TGGGCTA48150.09.8156752
GTCCTAC51050.09.5138986
Produced by FastQC (version 0.11.5)