FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_Neg.10.30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_Neg.10.30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4256378
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC1101092.586917797244512Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217180.5102460354789917No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATA48630.11425207065725836Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC13500.042.9569059
AACAGAC15700.038.783435
GCATTAG21900.037.7335175
TTGTACT19900.034.2703931
AGCATTA25350.033.1696974
CATTAGA25600.032.2798466
TGTACTC21400.032.1872252
AGACATA22800.030.8392248
CCGTATA28750.028.246466145
CAGACAT25950.027.655067
TCTAGTT27650.026.7412577
TCCAACA23800.026.5042782
GTACGTT19150.026.125479145
CTCCAAC25600.026.0730691
GAGCGGG20300.026.0677539
CGGATGT11500.025.8660641
TCTATCA18800.023.5206187
CGTTCTA21050.022.7275874
ACCTTGT16950.022.6658886
CAGCATT38750.022.635253
Produced by FastQC (version 0.11.5)