FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_Neg.10.26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_Neg.10.26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6018948
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC601880.9999754109854413Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146250.24298266075732838No Hit
CCGGAATCTGGTGATTCATAATAACTTTCGGATCGACCTCGGTCGATGCA68640.11403986211543943No Hit
CTCCAACAGACATACTCCCCGAAGGAAGTGCAAGATGCGTTCAAAGTTTT65110.10817504985921127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA28000.049.202457145
GACATAC24750.047.4493189
AACAGAC26650.045.4250075
CGGATGT19900.041.195981
TGTACTC28600.038.0316622
TTGTACT28050.038.020161
GTACGTT22800.034.664333145
TCTAGTT35150.033.615667
GTCTTGG19850.033.232016
TCTTGGC19600.032.5466277
AGACATA46650.030.7680598
CGGTGGG40200.030.48259145
ACGTGTT23050.029.884354145
TCTCGGG31550.029.876877145
CTCCAAC45100.029.1159881
CAGACAT49800.028.6758467
TACTCTA41300.028.2588944
TCCAACA44700.027.7401432
GATGTCT29700.027.0934263
ATGTCTT25400.026.541694
Produced by FastQC (version 0.11.5)