FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_9424.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_9424.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14976200
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGATC118100.033.9507332
GATCCTG107150.032.4718485
ATCCTGG118100.030.0752986
CCTGGCT113550.029.8125618
TTGATCC114550.029.6166733
TGATCCT120000.029.4780924
TCCTGGC126350.027.5381057
CTGGCTC149250.026.1302329
TTGAACG69750.022.86377
TTGGTCC62300.022.6887366
CAATTAG45150.022.637939
TCAGATT70450.022.5391252
CATTGTA206100.021.987875145
CAACATA46800.021.8390674
GCTCAGA60200.021.6737445
GGCTCAG140200.021.2497924
AACGTAT375600.020.650096
CTCGGTT123050.020.6346381
CCATTGT281700.020.591328145
TGGGGAT167250.020.3320262
Produced by FastQC (version 0.11.5)