FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_8724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_8724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13638348
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC139930.10260040292270002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA54650.041.1299972
TCTAACA71650.031.058155
AGTCTAA72150.030.6445673
TGCCAGT41900.028.3802992
GATCCTG45800.027.8548345
AACGTAT155150.027.2379846
TAGCATA44700.026.9195798
GTTACGA45400.026.664094
GAACGTA159200.026.6358285
GTCTAAC83900.026.5242184
GTAGCAT45600.026.3881767
TTACGAC45600.026.2284415
GCCAGTA47350.026.026693
TACGACT48050.025.0422236
TGTGTAT21000.024.86023145
CCGTATA12750.024.454016145
ACGTATT178300.024.189777
CGTATTC180850.024.0892738
CTTGTTA52650.022.8731
TGGATGC54600.022.8363043
Produced by FastQC (version 0.11.5)