FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_8721.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_8721.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20922057
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA381880.18252507389689265No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC277950.1328502259600956No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT233460.11158558644592165No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT233120.11142307852425792No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT220110.105204760698243No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC211260.10097477509023133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG67450.042.665215
TCCTGGC68850.040.1137857
AGTCTAA105950.037.973373
ACTTCCT103450.035.1756136
TTACTTC102150.035.126364
CCTGGCT79150.034.4359138
CTTGTTA47000.033.3399471
GAGTCTA128000.031.5531272
GTTACGA54100.029.7455544
TCTAACA136500.029.5792985
TACTTCC123600.029.5581575
GCTCAGA66750.029.3209345
TGCCAGT52800.029.2437323
CCTGCCA53300.028.8547731
GTAGCAT55700.028.7615158
GTGTCGA38200.028.4638924
CTCGGCT31950.028.382341
CTTTTAC137300.027.4752621
TAGCATA58950.027.2994049
CTGCCAG56750.027.2151622
Produced by FastQC (version 0.11.5)