FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_8336.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_8336.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16222080
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA42350.031.1505054
GATCCTG105650.024.492995
TCGATCT61700.020.7951473
TGATCCT135200.019.4079194
AACGTAT328450.019.3761885
CTATTAG101400.018.3717428
CGTATTC352400.017.8746557
CGGGAAC301700.017.313171
GAACGTA393400.017.282864
TATTCAC370750.017.2053439
GTATTCA373650.016.9357478
CTGGCTC150150.016.800259
ACGTATT395450.016.4417976
ATCTATT93600.016.107796
TCCTGGC163050.015.9153457
GGAACGT345600.015.248763
AGTGTAT55850.015.060053145
TTGATCC191450.015.0317333
CCATTGT293550.014.943937145
CCTGGCT172100.014.7836418
Produced by FastQC (version 0.11.5)