FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_7920.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_7920.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21362175
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA34800.052.50522145
GTAGATA231550.029.121874145
CGTCGTT34700.028.626764145
AGATTAT330750.027.227194145
GAGCTCG87350.020.8293178
AGCTCGC91700.020.5531029
TCGTGCG33800.019.730337
AGTGTAT171000.019.03844145
CGTGCGT34700.018.3830438
TCGTGTT94250.017.924858145
ATTAGTT247700.017.856031145
TGCGCGA39600.017.3900899
GGCTCGT41650.016.533344
TGTTAAT300150.016.499224145
CGCGTAA26950.016.1507741
ACTAACC121900.016.1149437
AGGCGTA51500.015.4828068
TCGATCG40050.015.203418
CGTATTC58550.014.9803758
TACGACT23100.014.1207796
Produced by FastQC (version 0.11.5)