FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_7651.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_7651.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16172811
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA22200.031.681625145
CGTCGTT20650.024.930271145
AGTGTTA189100.023.849955145
CGTGTAT74700.022.325228145
GGCTCGT27000.021.2092724
TCGTGTT64450.020.138115145
AGCTCGC72500.019.6973049
CGTGCGT28050.019.640388
TCGTGCG28900.019.3135497
TTCGCAC27150.018.6894878
GAGCTCG76350.018.5137658
ATGTCCG26700.018.189726
GTAGATA141200.017.767654145
CAACCGA64250.017.261594
TGCGCGA34700.017.130229
CCAACCG60350.015.495653
ACTAACC94150.015.0135547
GAACGCT57000.014.7523679
CCGGCAT24700.014.3923261
AACCGAG72900.014.3184535
Produced by FastQC (version 0.11.5)