FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_7379.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_7379.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15752421
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG91100.056.5729265
TGATCCT103150.048.6291924
CCTGGCT110450.046.072158
TTTGATC142150.039.989432
TCCTGGC135600.036.832117
CTGGCTC141050.036.3348249
TCGATCT12250.029.5884133
TTGATCC173950.029.2550243
CGATCTA12650.028.6509914
ATCCTGG200550.026.2046456
GCTCAGA50350.024.474115
TCAGATT58550.023.157452
AACGTAT358050.023.139825
ATTGAAC72950.022.9535396
GGGCACA18100.022.0437221
GTATTCA386100.021.3465528
CGTATTC389300.021.1710857
GCTATAT120550.021.111816145
GAACGTA431200.019.903674
GATGCCT162700.019.6921045
Produced by FastQC (version 0.11.5)