FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_6852.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_6852.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18079096
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA91300.020.0068865
CGTATTC93800.019.7062458
TCGTGTT87500.018.810318145
AACGTAT102000.017.7661466
GGAACGT104000.017.6335814
CCGTATA19900.017.48906145
CGGGAAC107650.017.445592
AGAGCGG105450.017.396114145
ACGTATT109800.016.8344767
CCGGGAA117900.015.8136451
GGGAACG129050.015.39110853
CGTCGTT27900.012.994038145
GCGTCGG84200.012.572425145
ATGACGT44350.012.0948327
GACGTAC52050.011.25600590-94
CGGAAGC118950.011.0329685145
CGCGATT83250.010.81516730-34
TCGCGAT59500.010.72164930-34
TGACGTC47550.010.671228
CGGGGTT131850.010.6191521
Produced by FastQC (version 0.11.5)