FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_6331.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_6331.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13938888
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT63850.059.503937145
AGTCTAA63750.032.9744725
GGATCTC34250.029.849712
AGTGTAT82600.027.914024145
GATCATG63050.026.0976165
CTGGCTC100400.026.0645779
GTTTGAT187100.023.8469221
GATCCTG118550.023.7241255
TGATCCT129200.023.619734
TTTGATC185450.022.7941442
GAGTCTA87650.022.3288964
TCTAACA102650.020.9736067
TCCTGGC131400.020.3566657
TTGATCA82450.020.1338423
CCTGGCT127950.019.6591538
TAACATG47150.019.2178829
TCTCTTG55650.019.0172525
GTCTAAC122750.018.1885096
GGGAAAA68000.017.806679145
ACATAAC55550.017.4859246
Produced by FastQC (version 0.11.5)