FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_6119.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_6119.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16568396
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAAGT439010.2649683167881791No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAA338120.204075276810139No Hit
CCCCCTTTCACCCGTAGGTCGTATGCGGTATTAGCTTGGGTTTCCCCAAG282340.17040877101199176No Hit
CTAAGTTAGAACCCCAACATAACCAGGGTGGTATTTCAAGGTTGGCTCCA268410.16200119794336157No Hit
CGGGGCTCAAACTAGGAGCCGAAGCTGCGGATTTAATTGTTTCAATTAAG226410.1366517314047781No Hit
CCCCAACATAACCAGGGTGGTATTTCAAGGTTGGCTCCACAAATACTGGC214710.1295900942976013No Hit
CCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGGAGCTGCCTTCGC191930.11584102649405531No Hit
CTGATACTGGATAACTAGAGTAGGTGAGAGGGGAGTAGAATTCCAGGTGT185230.11179718302242413No Hit
CCGGGAACGTATTCACCGCAGCATTCTGATCTGCGATTACTAGCGATTCC182530.11016757445922948No Hit
CGGGAACGTATTCACCGCAGCATTCTGATCTGCGATTACTAGCGATTCCG174370.10524253524601898No Hit
CCCCTTTCACCCGTAGGTCGTATGCGGTATTAGCTTGGGTTTCCCCAAGT173960.10499507616790424No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATG167600.032.8254975
ATGGCTC165950.032.105539
TTTGATC182600.032.004732
GAACGCT225150.031.873749
CAGATTG240650.030.7555873
AGATTAT232300.030.744278145
TCAGATT246350.029.6679692
TGAACGC251350.028.4931168
CTCAGAT260650.028.4161721
TCGGGCT53350.028.2688922
AGATTGA279700.027.703214
AGTGTAT92850.027.019136145
CCGTATA39200.027.004992145
TTGATCA213050.026.6418513
TTGAACG276400.026.48767
GATTGAA297000.026.4311185
CTAAGTT166550.026.2645681
GCTCCGA70450.026.2363874
CGGGCTC69850.026.0678311
CCCCCTT167750.025.773971
Produced by FastQC (version 0.11.5)