FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_5764.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_5764.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18720804
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT136400.018.923832145
CCGTATA25750.016.894585145
CGTCGTT25300.014.329234145
GTAGATA174000.013.292751145
CGGGAAC62350.013.2634171
CGATCTA17250.013.0262564
CGGGGTT138500.011.8894871
GAACGTA66500.011.1179714
TGGTCGC144950.010.804608145
CGGGAAT61850.010.6730551
CGGGGAT57850.010.658661
CGTGTAT53700.010.261546145
CGTATTC63050.010.2321538
CTCGGTG224650.010.134369145
TCGGTGG217850.010.051314145
AGAGCGG77400.09.836075145
AACGTAT70800.09.8283975
CCCGAAT73100.09.6259011
CGAGGAT42250.09.4432831
GTGGTCG160900.09.373047145