FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30908.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30908.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19037088
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTTA194200.019.900024145
TCGTGTT76950.019.221937145
CGTGTAT70600.018.58872145
CCGTATA26600.018.26286145
GTAGATA160350.013.972211145
CGTGTAA37450.012.197318145
TCGGTGG235650.011.999766145
CCGTGTT87400.011.199482145
CGTCGTT37900.011.095947145
TGGTCGC174700.010.832364145
CTCGGTG247100.010.710155145
AGAGCGG130200.010.69217145
CGTATCA83300.010.619166145
GAGCTCG87650.010.5032478
CGGGGTT145250.010.4389781
GTGGTCG180800.010.146066145
CGCCGTA102500.010.044772145
AACCGAG77850.09.9628165
CGAGGGA120300.09.8821178
CCAACCG69800.09.8662853
Produced by FastQC (version 0.11.5)