FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30907.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30907.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15958416
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTATT23550.029.8566576
CGATCTA28450.025.2236444
GATCCTG177500.023.3180735
TGTACGG51600.022.763905145
TGATCCT177300.022.404064
TGTCCTA62300.021.8738255
CTGGCTC177700.021.4165029
TTGATCC169750.021.0533223
TTTGATC175900.020.7764842
CTATTAG59100.020.7284958
TTAGACC65950.018.5751466
TCCTGGC195250.018.3401577
CCTGGCT205050.017.6757858
GTAGATA134100.017.518532145
GTTTGAT246350.017.374721
AAACTCG41950.017.280313
CTACTCT85650.016.8423379
AGATTCA88800.016.5705914
GATCTAT45800.016.4597345
GCAAACT47050.016.3442361
Produced by FastQC (version 0.11.5)