FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30907.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30907.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12349286
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG133350.029.253523145
GTCCTAC89350.026.853946
TGTCCTA95050.024.9381925
GCGGTGT104750.023.2707251
TCGTGTT98400.023.137873145
CCTACTC109600.021.8928648
CGGTGTC118450.020.5073342
CTACTCT119500.020.3827029
TCCTACT119900.020.254087
GTGTCCT131100.018.633444
GGTGTCC137950.018.2876763
TCTCGGG93400.018.243572145
CGGTGGG108800.017.460686145
ATGTGTT147400.016.921928145
AACGTAT63750.016.260376
AGAGCGG141750.016.061848145
GAACGTA69850.015.2551574
GTAGATA118650.014.5444765145
GTAGATG107950.014.038237145
CGTATTC76100.013.6218638