FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30907.TR1_batch_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30907.TR1_batch_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15318021
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC222650.14535167434487783No Hit
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG187410.12234609157410087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA92150.039.488525
GAGTGTG116650.029.401487145
GATCCTG125150.028.9602035
GTGTCCT127300.028.869594
TGGGCTA98200.027.3202442
GGAACGT250950.027.296424
CTACTCT140200.027.1966729
AACGTAT390450.027.0865556
TGTGTAT68400.027.031925145
CGATCTA38000.026.896554
GTATTCA411100.025.8678729
CCTACTC152650.025.8331478
ATCTATT44200.025.7478686
CGTATTC417450.025.4915858
CCGGGAA282000.025.3647561
GTCCTAC148950.024.9168626
GTAGATA238250.024.712463145
CGGTGTC148000.024.4474262
TGATCCT153850.024.028894
GGCTACA118100.023.9373344
Produced by FastQC (version 0.11.5)