FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30905.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30905.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979805
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA179480.11981464378207861No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG57950.045.655845
CGATCTA21900.040.7113044
TGTCCTA75750.035.0231635
TCGATCT25850.034.4923863
TGATCCT76350.034.083134
GTGTCCT83350.032.0903744
CTGGCTC93750.030.0000329
AGCAATT62450.027.62557
GGTTAGA133350.027.0157724
CGAGCAA64500.026.8593245
GCAATTA69250.026.3778328
TCAGATT60450.025.9082242
TTGATCA38200.025.8079913
GATCATG34400.025.2859785
TTGAACG61400.025.2645197
CCTGGCT106750.024.7846818
TTTGATC149050.024.566292
GAGCAAT70850.024.554676
GTTGGTC49850.024.4287385
TCCTGGC110550.023.9987147
Produced by FastQC (version 0.11.5)