FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30890.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30890.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19042622
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTCCTACTCTCCCACAACCTGTCGGTTGCAGTACCATTGGCGCTG200210.10513783238463693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA162350.032.7263035
GTCCTAC178500.030.9432186
CCGTATA62400.029.747553145
AGATTAT294250.027.796394145
CTACTCT214750.026.3620459
GTGTCCT206700.026.2657644
GTAGATA273800.025.953054145
TCCTACT224250.025.826897
CCTACTC227900.025.3814498
GCGGTGT220350.024.5617981
GGTGTCC225300.024.3239043
CGGTGTC215150.024.2645472
AGTGTAT123600.024.052557145
ATTAGTT163850.022.436617145
TGTTAAT213150.018.743994145
TCGTGTT138100.016.224102145
GAAACGC208250.016.1155344
CGATCTA40100.015.9069544
AAACGCC217500.015.2300815
CGGGGAA229150.014.9753031
Produced by FastQC (version 0.11.5)