FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30852.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30852.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15029711
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT199210.132544132086106No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT153050.1018316320253929No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG151650.10090014372199171No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA151460.10077372745224442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAA79950.041.7997133
GAGTCTA83550.039.9209142
TCTAACA89100.037.9107975
GTCTAAC102200.032.909764
CCTCAAT33400.032.146971
TAGTCAT56950.031.693739
CCTGCCA68700.030.8354911
GATCCTG50500.030.1427355
GTTACGA39300.029.3266324
GTAGTCA66100.028.4029278
GCCAGTA78700.028.0920034
CTGCCAG76650.027.7168942
TGCCAGT82900.025.8837993
AGTAGTC70200.025.711487
CAATTAG28200.024.6768579
TACGACT46750.024.3431666
GCTCAGA47650.023.8831425
GGATCTC23200.023.440262
GGAGTCT149950.022.9811571
CTCGGCT34900.022.8660961
Produced by FastQC (version 0.11.5)