FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n02_MT_30783.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n02_MT_30783.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17983446
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG101000.022.398623145
AGAGCGG109750.014.86504145
TCGTGTT109550.014.428866145
GCGTCGG99250.010.666217145
CGGGGTT130350.010.0172871
GTGGTCG169600.09.9613495145
CGGGGAT88800.09.88464451
TCGGTGG223150.09.585465145
GGGATAG121450.09.317851
CCGGGAA89100.09.0372011
GTGTAGA414150.08.981477145
CGGGAAC84550.08.8371511
TCTCGGT213000.08.748662145
CCTCTAT599500.08.575217145
GCGGGAT63050.08.5140321
CGGAAGC150000.08.459308145
ATCTCGG273650.08.452464145
GATCTCG278650.08.4309025145
GGGGGAT119400.08.3842341
GGGGAAT173750.08.3083911