FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20062148
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13247386.603171305485335No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251730.12547509867836684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG57650.017.1019575
CGAGGGA81350.014.7014468
TGTCCTA50750.013.9989745
CGGGAAC106850.013.4357812
GAACGTA124450.013.0484625
TTTGATC97700.012.7645682
GGAACGT113900.012.5386534
TGATCCT75850.012.2338394
GACGAGA62050.012.031619
CGCGCGA66400.012.0097224
GTATTCA138250.011.9011519
GTCCTAC54850.011.76318556
TTGATCC80100.011.5859693
CGATCTA22050.011.5071524
GTGTCCT62800.011.428364
AGTCTAA61300.010.8813373
CCGGGAA133450.010.7742861
TATTCAC137550.010.4335689
ATACGAG28950.010.2669525
ACACGTA71350.010.162181145
Produced by FastQC (version 0.11.5)