FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_Neg.10.30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_Neg.10.30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4256378
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT3043927.151432509048774TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA1179472.7710649759020463TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATATCGTAT91620.21525343848690132TruSeq Adapter, Index 7 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTA90380.21234016339714187TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTAGCTCGTAT43760.10281041768376775TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC14750.039.3215839
AACAGAC15100.036.9745525
GAATCGG43800.036.763861
CGGATGT10700.035.9281041
ATTCGTT17500.033.97144145
AATCGGA50600.032.098642
CGTCTTG32050.028.049929145
AGACATA25000.025.8097028
CTCCAAC26100.025.5675751
TCTTGGC14400.025.173327
GATGTCT15550.024.713523
TCTATCA16050.024.3922427
TCCAACA24800.023.9745982
CAGACAT27750.023.7745027
TAAGCGA14150.023.5719576
ACCTTGT16650.023.5165586
TAGCATA11400.022.2585148
GAGTCTA7300.021.8518892
GCTGAAT13550.021.4122811
CGGAAAC10950.021.197181
Produced by FastQC (version 0.11.5)