FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_Neg.10.26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_Neg.10.26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6018948
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1759672.9235507600331485TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA670771.1144306280765344TruSeq Adapter, Index 2 (97% over 37bp)
CCGGAATCTGGTGATTCATAATAACTTTCGGATCGACCTCGGTCGATGCA72600.12061908492979172No Hit
CTCCAACAGACATACTCCCCGAAGGAAGTGCAAGATGCGTTCAAAGTTTT71970.11957239039114477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAC22800.045.152179
AACAGAC23850.043.7791945
CGGATGT17850.041.0385781
CATTATG76150.037.512245145
CGTCTTG36950.030.805763145
TCTTGGC19350.029.5986047
AGACATA44000.029.1639068
CTCCAAC43200.028.7092291
GTCTTGG22200.028.4155676
TCCAACA40550.027.5376642
CAGACAT49200.026.6709567
GATGTCT26500.026.5413593
TGTCTTG24300.025.9601175
GAGTCTA11250.025.7810824
GGATGTC29850.025.7489072
GGAGCGT14800.024.9865172
ATGTCTT25600.024.641834
TTGTACT26650.024.4937021
TCTAGTT34500.023.3253967
TGTACTC29000.022.503072
Produced by FastQC (version 0.11.5)