FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_9424.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_9424.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14976200
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG102650.037.717895
TTTGATC119900.037.068872
TGATCCT115950.033.6417124
CCTGGCT115800.032.86878
TTGATCC116250.032.432353
ATCCTGG121400.032.1911626
TCCTGGC129200.029.7402847
CTGGCTC154100.027.522329
CAATTAG47100.026.4752049
TCAGATT69800.024.2029632
CATTATG96950.023.556013145
GCTCAGA63500.022.9502535
GGCTCAG143250.022.3208394
CAACATA41150.022.2007314
TTGAACG74350.021.6473037
AACGTAT385800.021.3680466
CTCGGTT131200.021.1716541
CATTGTA212850.021.15227145
TTGGTCC66500.021.1517686
GTATTCA405300.020.4098979