FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_8724.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_8724.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13638348
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC148160.10863485812211274No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT145150.10642784595319021No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA56400.041.781992
AGTCTAA71200.032.68953
TCTAACA72900.032.125885
TAGCATA44950.029.3543458
TGCCAGT46150.029.2230852
GTAGCAT46150.028.7481617
GTCTAAC85150.027.5042344
GCCAGTA52050.026.0497863
AACGTAT150400.025.695836
GAACGTA152850.025.1888965
GTTACGA47850.025.1540434
GATCCTG43650.025.0826135
TCCAGTA33450.024.925665145
TTACGAC48600.024.6165835
CTCGGCT37200.024.1757431
GACATAC20150.023.7464989
TACGACT51100.023.412426
TGGATGC57300.023.2834013
CTGCCAG60100.022.9287411
CGTATTC177050.022.4396118
Produced by FastQC (version 0.11.5)