FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_8721.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_8721.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18687621
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG676490.36199899387942425No Hit
CCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTAC525410.2811540323939575No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC517010.2766590782208179No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG514400.27526243174559245No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG468570.25073817582237995No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT443940.2375583280504244No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC378820.20271173093675218No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT373690.19996659820958484No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA371630.19886426420998157No Hit
CCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGC357750.19143688755246052No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA350590.1876054742334511No Hit
GTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAG341760.18288042121573422No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGAT335900.17974465556637734No Hit
GCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAA333350.17838011590667427No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA296210.15850599709829302No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC281170.1504578886740051No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA279140.14937160808216307No Hit
GGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAG273440.14632146060753268No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA262080.1402425702019535No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC252620.13518039562125111No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT245560.1314024936614457No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC244130.13063728122482793No Hit
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC242320.12966872562323475No Hit
CGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGC240470.12867876547795998No Hit
TGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCA239440.12812759847815836No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC235380.12595503729447424No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC234310.125382465750991No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT228280.12215573079098725No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG227550.12176509786879774No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG227460.12171693764551411No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA226600.12125673995635936No Hit
CGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGG222320.11896645378242635No Hit
TGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA219380.1173932198218275No Hit
CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC217060.11615175628829372No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC212630.11378120307555467No Hit
CCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCG211840.11335846333784273No Hit
TCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACTAGC206150.11031366699913274No Hit
GCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCG203480.10888491370838482No Hit
CTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAG202540.10838190693186682No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT201390.10776652630102035No Hit
CCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGC198880.10642339118499888No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG196590.10519798105922631No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG194500.10407959365186184No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA194380.10401538002081699No Hit
GTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCC191750.10260803127375069No Hit
CTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACC190400.10188562792449612No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTG188350.10078864506081325No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT186960.10004483716787706No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACTA186000.045.2575764
CTAACCA193600.042.8401378
TCCCTGA197500.042.5123023
AAGAAAC198850.042.2236862
AGAAACT202150.041.498543
CTCCCTG204550.040.6571662
CTCGGCT58100.036.4497831
TGTGTCG39350.036.1147423
CGGTCAA26750.035.5074544
CCCTGAG243900.034.840784
TGTCGAG41350.034.192525
CTGAGCT262250.033.5096
CCTGAGC257400.033.4923255
TAACCAG251750.033.4631549
AACTAAC252700.033.3981256
GTCGAGG44000.033.122096
AGGCGTA53350.032.8860058
CGCTCAG46600.032.8298234
CGTGCGT58650.032.386468
GAGCTCG277000.031.8784878
Produced by FastQC (version 0.11.5)