FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_8721.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_8721.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20922057
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA384300.18368174792755798No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC290030.1386240368239127No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT253040.1209441308758503No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT233500.11160470502494091No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT231410.11060575927118448No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC224370.1072408893637944No Hit
CCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTC210540.10063064066788462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG65000.043.0579265
AGTCTAA103550.042.9231573
TCCTGGC68950.040.060757
CCTGGCT76700.035.4457558
GAGTCTA126700.035.2521322
TTACTTC100750.033.3927464
ACTTCCT103750.032.56716
TCTAACA140600.032.385725
GTAGCAT54300.030.1742828
TGCCAGT52000.029.7000733
GCTCAGA67050.029.3055365
CTTGTTA50750.029.0103761
GTCTAAC156650.028.8361194
TAGCATA57600.028.6972779
GCCAGTA55100.028.686934
GTGTCGA40200.028.6780914
CCTGCCA53100.028.4094091
TGTCGAG41950.028.3459555
GTTACGA54150.027.985114
TACTTCC122850.027.9757925