FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_8336.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_8336.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16222080
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA42100.037.2007184
GATCCTG102800.026.7314875
TCGATCT63200.025.2398433
CTATTAG103250.020.7839988
ATCTATT92100.019.6814656
TGATCCT136950.019.5363774
AACGTAT318200.017.9101585
GATCTAT102600.017.0312585
CGGGAAC296650.016.821021
CTGGCTC152700.016.7121059
CTCGATC99250.016.3643932
CGTATTC351050.016.1084277
TATTCAC364700.015.8037039
TCCTGGC171300.015.7439277
GAACGTA382200.015.6700024
GTATTCA367850.015.4909938
ACGTATT394000.014.7589416
TTGATCC192100.014.75813
CCTGGCT175900.014.7139718
TATTAGT150250.014.66852959