FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_7379.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_7379.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15752421
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATGCAA159530.10127332173257686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG93650.058.3782585
TGATCCT108350.049.922794
CCTGGCT113900.047.7381748
TTTGATC145400.040.244352
TCCTGGC140400.038.5728537
CTGGCTC145250.037.5344439
TTGATCC183250.030.1116893
CACATTA38700.029.037462145
ATCCTGG201200.028.3979646
CGATCTA14750.026.0539024
TCGATCT15300.024.6437243
GCTCAGA49700.023.926275
GGGCACA17600.023.9018291
CAATTAG46250.022.5724669
CTCGGCT46650.022.3885861
AACGTAT358950.022.3817165
GGCCAAC43050.021.0597231
GTATTCA392150.020.6874128
CGTATTC396800.020.444987
ACGAGCA41750.019.798734
Produced by FastQC (version 0.11.5)