FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_6852.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_6852.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18079096
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATG86000.022.256176145
GAACGTA89200.017.882945
CGTATTC96050.016.8319538
AACGTAT99250.016.6565656
GGAACGT97850.016.3020784
CGGGAAC103300.015.5825082
ACGTATT106450.015.1193917
CCGGGAA112850.014.8466911
GGGAACG124750.014.007443
ATGCCGG47700.011.855538145
CTACGCG33200.011.355606145
CGGAAGC94700.011.177585145
GACGTAC50800.010.4467690-94
CGCGATT80300.010.29288230-34
TCGCGAT56400.010.12965930-34
GGGATAG117700.010.1061711
TCGTATG257850.010.0660925145
CGTCTTG53300.010.065829145
ATCTCGT314550.010.049439145
TGCCGTG66150.09.973706145