FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_6331.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_6331.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13938888
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAA64400.032.874935
GGATCTC33300.027.2167842
CTGGCTC102400.026.4790339
GAGTCTA83000.024.45964
GATCCTG112500.024.2327795
GATCATG58900.023.88115
CATTATG92050.023.786087145
TGATCCT129150.022.7368374
TCTAACA100750.022.5231847
GTTTGAT192500.021.8894481
TTTGATC187350.021.8658182
TAACATG42300.021.5953719
CCTGGCT126950.020.5018128
TCCTGGC134350.019.5884237
CTAACAT95100.018.448688
GTCTAAC124700.018.4316336
TTGATCA80300.017.878073
TCTCTTG52650.017.7647555
ATTGTGT29000.017.7513486
CTCATTT32500.017.623152145
Produced by FastQC (version 0.11.5)