FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_6119.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_6119.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16568396
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAAGT455970.27520467279995No Hit
GTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAA348470.2103221096357185No Hit
CCCCCTTTCACCCGTAGGTCGTATGCGGTATTAGCTTGGGTTTCCCCAAG309020.18651171785126333No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT298300.18004156829665346TruSeq Adapter, Index 7 (97% over 35bp)
CTAAGTTAGAACCCCAACATAACCAGGGTGGTATTTCAAGGTTGGCTCCA247140.14916350381775037No Hit
CCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGGAGCTGCCTTCGC208840.12604720456947072No Hit
CCCCAACATAACCAGGGTGGTATTTCAAGGTTGGCTCCACAAATACTGGC196460.11857514752785968No Hit
CTGATACTGGATAACTAGAGTAGGTGAGAGGGGAGTAGAATTCCAGGTGT191940.1158470620813264No Hit
CCCCTTTCACCCGTAGGTCGTATGCGGTATTAGCTTGGGTTTCCCCAAGT183510.11075906201179644No Hit
CCGGGAACGTATTCACCGCAGCATTCTGATCTGCGATTACTAGCGATTCC180590.10899667052863779No Hit
CGGGGCTCAAACTAGGAGCCGAAGCTGCGGATTTAATTGTTTCAATTAAG175770.10608751746397176No Hit
CGGGAACGTATTCACCGCAGCATTCTGATCTGCGATTACTAGCGATTCCG175130.10570123987862193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCATG166650.034.0678635
ATGGCTC164350.032.8634729
TTTGATC181500.032.5191272
CAGATTG231850.030.179363
GAACGCT225000.030.0947849
TCAGATT238750.029.2465062
TGAACGC248400.027.4932588
AGATTGA273050.027.2188784
CTCAGAT257600.027.084611
TTGATCA214350.026.8588143
TCGGGCT53350.026.6386832
CCCCCTT183700.026.491511
TTGAACG266050.025.751087
GATTGAA287050.025.5377185
CCCCTTT225300.024.5880032
CTAAGTT160550.024.5291731
CGGGCTC65700.023.9545351
CCCTTTC253400.023.864333
GCTCCGA66600.023.2985064
TAAGTTA172000.022.5957852
Produced by FastQC (version 0.11.5)