FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_5764.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_5764.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18720804
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG80850.022.68751145
TACTCGC39750.017.327377145
TCGATCG25350.013.728074145
CGGGAAC62950.012.5580821
GAACGTA69550.012.0930284
CGTATTC63950.011.7901837
TATGCCG245750.011.41731145
AACGTAT68550.011.31755
GCCGTCT212200.010.76246145
CGTATGC295100.010.294166145
GGAACGT70100.010.2398523
ATGCCGT239900.010.184648145
GGGAACG82250.09.9613692
GGGGAAT207750.09.7748371
ACGTATT84000.09.753846
GCGAAAT42550.09.7155591
GTATGCC307400.09.622827145
CGGGCAT62900.09.5701761
CGATCTA16157.8216544E-119.428034
CTCGTAT296550.09.388142145