FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30908.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30908.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19037088
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATG135100.019.158434145
TACTCGC46900.017.932148145
CGAGGGA109150.012.9521238
ACACGTA48850.012.021749145
GCCGTCT251700.011.925161145
TCGTATG364600.011.6925125145
GAGCACC130050.011.3170395145
CTCGTAT341300.011.194933145
CGGGAAT56650.011.1381071
TATGCCG292450.011.056824145
CGTATGC338550.010.986054145
ATGCCGT285000.010.608119145
TGCCGTC269100.010.561353145
ATCTCGT399700.010.484335145
CCAACCG59400.010.3752353
AGCTCGC98600.010.293939
AACCGAG71400.010.2562235
GGGATAG112150.010.0206684
CAACCGA70300.010.0041624
GGGGATA117750.09.8520023
Produced by FastQC (version 0.11.5)