FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30907.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30907.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15958416
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGATG63450.038.04992145
GATCCTG177100.024.7279475
TGATCCT177400.023.623634
TGTCCTA61000.023.1779175
ATCTATT23650.022.9932926
TTTGATC175100.022.5676772
TTGATCC169250.022.3623943
CTGGCTC186000.021.7106329
TCCTGGC202450.019.6600577
CTACTCT78250.018.993279
CGATCTA27350.018.822334
CCTGGCT208500.018.6723258
GTTTGAT244900.017.7987751
TTAGACC67750.017.444136
CTATTAG57350.017.3187838
AGATTCA84600.017.0551344
AAACTCG38200.016.8928573
AACGTAT249000.016.277295
CGTTAGA69300.015.7985094
GCAAACT43000.015.5175721
Produced by FastQC (version 0.11.5)