FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30907.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30907.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12349286
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT237280.19214066303104488TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC84850.028.2849966
TGTCCTA92800.025.6274745
CATTATG128750.024.157171145
GCGGTGT102700.023.6579691
CCTACTC110850.022.3021978
TCCTACT120700.020.902637
CTACTCT119950.020.368489
CGGTGTC117150.020.362782
GTGTCCT127850.018.828574
GGTGTCC133200.018.6168163
AACGTAT66450.016.149046
GAACGTA72800.015.1388255
TGCCGTG61000.013.66797145
ACGTATT82750.013.4045557
AAACGCC89250.013.1609355
CGTATTC85750.012.9355918
GAAACGC89350.012.5781594
GGAACGT72100.012.5705624
CGGGAAC80200.012.4763692
CGTCTTG58600.012.000797145
Produced by FastQC (version 0.11.5)