FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30907.TR1_batch_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30907.TR1_batch_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15318021
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCC222250.14509054400695756No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT195510.12763398091698658TruSeq Adapter, Index 19 (97% over 38bp)
CGGGAACGTATTCACCGCAGTATGCTGACCTGCGATTACTAGCGATTCCG193810.12652417698082538No Hit
CGAGAACGTATTCACCGCAGCGTTGCTGATCTGCGATTACTAGCGACTCC153750.10037197363810899No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA96500.036.6661875
AGCTATG145450.033.44695145
CGATCTA39350.030.9554984
GATCCTG129950.028.567155
ATCTATT45150.028.4242346
GTGTCCT130650.027.3041694
CTACTCT139100.026.9978479
AACGTAT385650.026.3025366
GGAACGT248900.026.2757344
GTATTCA409550.024.9242579
CCTACTC154950.024.7976638
CGTATTC417750.024.4350198
TGATCCT155300.023.7173144
GTCCTAC150850.023.6479246
CGGTGTC150650.023.486882
CCGGGAA285100.023.3019371
TGGGCTA101200.023.2134232
CTATTAG66850.023.099618
GGGAACG299750.022.568213
CGGGAAC299200.022.3673632
Produced by FastQC (version 0.11.5)