FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30905.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30905.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979805
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGTTAGAACCTCAAAGACACCAGGGTGGTATTTCAAGGACGGCTCCA157180.10492793464267393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA21200.048.224214
GATCCTG59450.044.15085
TCGATCT27050.036.991293
CACATTA54900.033.93946145
AGCAATT64700.032.4952967
TGATCCT80000.032.356394
TGTCCTA76600.032.2780655
GCAATTA72250.031.106468
GTGTCCT82400.030.534034
GTTGGTC48450.030.0804355
CGAGCAA70100.029.9958975
GATCATG32850.029.7975315
CTGGCTC96300.029.6618449
CAATTAG82550.027.4009599
GAGCAAT77400.027.2605956
TTGATCA37150.027.1296143
TGACCTA108750.026.8659738
CGATGAC87900.026.066345
CTCGATC40850.025.9149232
TCAGATT61350.025.5286772
Produced by FastQC (version 0.11.5)