FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30890.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30890.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19042622
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGTCCTACTCTCCCACAACCTGTCGGTTGCAGTACCATTGGCGCTG204140.10720162380999843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA160400.032.0038035
GTCCTAC182900.029.0975346
CTACTCT212200.026.067989
GTGTCCT201550.025.5776524
TCCTACT220000.025.1765737
CCTACTC228550.024.3298878
CGGTGTC210100.024.1921122
GCGGTGT224900.023.7671621
GGTGTCC222450.023.7615433
GAGAATG381550.019.457651145
CGATCTA40800.018.1264954
GAAACGC203950.015.2157684
AAACGCC212400.014.3373045
AGTCACA115300.013.644962145
AACGCCC227850.013.3969796
GAGCACC177250.013.007169145
CGGGGAA238000.012.616031
TGGCGGT163850.012.5267751
AACGTAT128000.012.518616
GTACCAT219600.011.97763135-39
Produced by FastQC (version 0.11.5)