FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30852.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30852.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15029711
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT199500.13273708323466765No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT168070.11182517082331124No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA166050.11048116627126096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAA79050.043.0167273
GAGTCTA81950.041.75992
TCTAACA87350.039.344045
CCTCAAT31600.036.4937171
TAGTCAT55200.035.7240039
CCTGCCA71050.033.7887341
GTCTAAC105050.032.6460044
GTAGTCA64400.031.5210748
GATCCTG52600.030.8763375
CTGCCAG80600.030.3152582
GCCAGTA84100.029.7432254
AGTAGTC67750.029.5344317
GTTACGA41500.029.176514
TGCCAGT88250.027.769523
CAATTAG27650.027.5311579
ACCATTA43950.026.7230729
GGATCTC23150.026.3084182
TACGACT48700.024.5651826
GCTCAGA47100.024.1680875
TTACGAC51650.023.863945
Produced by FastQC (version 0.11.5)