FastQCFastQC Report
Tue 4 Dec 2018
HG22CBGX9_n01_MT_30783.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG22CBGX9_n01_MT_30783.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18660725
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT467940.2507619612849983TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA34050.026.1918074
TGTCCTA106700.023.3760075
GTCCTAC122050.020.554926
ATTCGTT106150.020.285076145
CGTCTTG94300.019.605085145
TCCTACT134900.019.239717
TGCCGTG127050.017.747042145
CGGTGTC143950.017.679812
GTGTCCT144700.017.1871194
CTACTCT157900.016.3454789
TCTCGTT126000.015.996114145
CCTACTC168900.015.6672158
TCGTATA132300.014.905594145
GCGGTGT204450.014.8998811
GGTGTCC174300.014.3516783
CTATTAG82850.013.8258928
AACGTAT156550.013.4777145
CGTATCG107350.012.764387145
CCGGGAA142850.012.3380551
GGATCCT93400.012.1879394
Produced by FastQC (version 0.11.5)