FastQCFastQC Report
Wed 13 Feb 2019
HFYKYAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYKYAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15655687
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG1427310.9116878741891046No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1396770.8921805858791121No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1305400.8338184073301925No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1215280.7762546606865608No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1107910.7076725537499569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC158950.0144.15936
CTGTATC159600.0143.798864
ACTGTAT159450.0143.707243
CGATAGG122500.0143.347066
CAATGGT146100.0143.12084
TATCTCT160350.0143.072927
ACAATGG146250.0142.9743
ATACAGG136950.0142.901037
TCGCATC152950.0142.710537
ATGGTCT146500.0142.631266
ACGATCG123600.0142.131261
GATACAG137950.0141.925756
CGCATCT153800.0141.874778
GCATCTT154150.0141.599589
AATGGTC148050.0141.040245
TCGATAG124850.0140.938665
AACAATG148600.0140.909992
CGATACA138800.0140.900245
ATCGAAT1200.096.470356
GTCTAGT1450.089.812069