FastQCFastQC Report
Wed 13 Feb 2019
HFYKYAFXY_n01_CAS13D.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYKYAFXY_n01_CAS13D.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19130896
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC419854421.946405437570725No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG417159021.8055129252702No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG382921220.015852890528492No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT310084616.208576953217456No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC214545111.21458712649946No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCTACCAACTGGT464330.24271210297729914No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCTTACC271250.14178635438716514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT3347100.0145.014081
ATACTGT4163700.0144.929231
TACTGTA4169550.0144.790632
ACTGTAT4173300.0144.686513
CTGTATC4179400.0144.52394
TCGATAC4583750.0144.489264
ACGATCG2325950.0144.48271
TGTATCT4181950.0144.468725
GATACAG4584450.0144.456126
GTATCTC4179950.0144.399086
ATACAGG4590250.0144.389837
CGATAGG2327700.0144.318166
CAATGGT3358300.0144.310154
ACAATGG3358450.0144.308013
TACAGGT4595650.0144.209148
TATCTCT4184750.0144.123027
GGTCGCA4445250.0144.11425
CGATACA4591800.0144.0975
TCTCTTG4180050.0144.026649
ATGGTCT3366400.0143.8946