FastQCFastQC Report
Wed 13 Feb 2019
HFYKYAFXY_n01_CAS13D.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYKYAFXY_n01_CAS13D.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22407796
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC438334919.561714146273022No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG434391319.385721826457182No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT425271718.978738471199936No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC396721617.70462387286996No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG361674016.140543228794122No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAT275090.12276530900227758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT4539150.0145.019641
ATACTGT3864350.0144.964741
TACTGTA3871800.0144.735522
ACTGTAT3874250.0144.609533
CGATAGG4286300.0144.426446
GATACAG4725750.0144.423936
TCGATAC4727050.0144.41954
AACAATG4565000.0144.36812
ACAATGG4557800.0144.362583
ATACAGG4731050.0144.32967
TGTATCT3884800.0144.315735
CAATGGT4560250.0144.312064
AGGTCGC4669950.0144.298954
GTATCTC3884450.0144.259666
ACGATCG4294550.0144.225251
TACAGGT4735950.0144.145058
CGATACA4732700.0144.098485
GGTCGCA4678950.0143.982645
ATGGTCT4574000.0143.946386
TCGATAG4307300.0143.936085
Produced by FastQC (version 0.11.5)