FastQCFastQC Report
Wed 13 Feb 2019
HFYKYAFXY_n01_CAS13D.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYKYAFXY_n01_CAS13D.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19296176
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG463846724.038270587913377No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT391850420.30715308566837No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC348645118.068092869799695No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG338505517.542620879909055No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC218502111.32359592905869No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCAAACCCCTACCAACTGG228740.11854162192550483No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGGT213840.11081988472741956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAT4227550.0145.043751
ATACTGT4982800.0144.896181
TACTGTA4989200.0144.734192
ACTGTAT4991850.0144.638343
ACGATCG3779100.0144.5241
CTGTATC4997750.0144.482094
CGATAGG3782000.0144.458026
TGTATCT4999950.0144.457665
GTATCTC4998900.0144.403886
ACAATGG4246800.0144.315293
CAATGGT4246850.0144.294824
GATACAG2365750.0144.271556
ATACAGG2369050.0144.21477
AGGTCGC3673350.0144.153534
AACAATG4257400.0144.132462
TAGGTCG3675850.0144.07723
TCTCTTG4998600.0144.053929
GGTCGCA3676600.0144.049775
TCGATAC2370600.0144.031454
TATCTCT5011450.0144.012957