FastQCFastQC Report
Thu 17 Jan 2019
HFYHHAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFYHHAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38340262
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG820600.21403087960118794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA192500.052.9263154
GTCCTAT325700.052.828045
GGCGCTA132500.051.6141624
AGTCCTA340350.050.3986744
GTCCTAA299450.049.0318225
AGGTCCT207750.048.6868743
GCGCTAT149500.048.3447465
GATCCTA402550.048.3235634
GCGCTAG115650.048.210385
CGCCAGA242350.047.9475676
ATCCTAT546350.047.8734175
TGTCCTA296650.047.6293454
GTCCTAG195150.047.3663865
TCCTATA475850.047.2211466
CGATCCT228150.047.1254843
GCGCCGC176650.046.9004672
GAGTCCT245450.046.3705863
TCCTATG299100.046.035146
TAGTCCT270500.045.983843
GCGCTCA141250.045.816185
Produced by FastQC (version 0.11.5)